To further confirm the correlation between MELK and MYCN/MYC expressions in NB, we overexpressed MYCN with a 3XFLAG tag in MYCN-amplified IMR-32, NGP cell lines, and MYC with a 3XFLAG tag in MYCN-non-amplified SK-N-AS, SH-SY5Y NB cell lines. This evidence concerns the gene MYCN and neuroblastoma.