To evaluate the correlation between MELK and MYCN/MYC expression in NB cells, we performed an sh-RNA mediated knockdown of MYCN in two MYCN-amplified NB cell lines, NGP and IMR-32, as well as knockdown of MYC in two MYCN-non-amplified NB cell lines, SK-N-AS and SH-SY5Y. This evidence concerns the gene MYC and neuroblastoma.