BMI1 and malignant colon neoplasm: When 100 pmol of control miRNA, miR-15a or Mimic-1 was added to colon cancer cells growing in 2 ml regular growth medium (50 nM), we found that protein expression of target genes YAP1, and BMI1 was reduced in cells three days after treatment with Mimic-1, but not in cells treated with miR-15a (Figure 8A).