When comparing the CRCs against localized PCa (samples 4201_LCM, P430_LCM and P4501_LCM) and CRPC (TURP1_LCM_#3, TURP1_LCM_#4 and TURP2_LCM), the TDCM cells clustered with the localized PCa samples while a subset of the proteins in the conventional cultures (e.g. total mTOR, Jak Y1007, PTEN S380) clustered with the TURP CRPC samples (Figure 9). Here, MTOR is linked to posterior cortical atrophy.