TIAM1 and neoplasm: Moreover, consistent with the Tiam1 expression, Rac1 and c-Myc expression was much higher in the 3D-cultured EL4 cells (2.1-fold and 2.3-fold, respectively) and fresh EL4 tumor (2.2-fold and 2.4-fold, respectively) than the 2D-cultured EL4 cells (Figure 6A).