To determine which signaling pathway is related with the protective effect of TRAIL on CAC, we analyzed the differential gene expression in acute colitis samples treated with AOM/DSS for 6 days with/without TRAIL using the Agilent mouse 8 × 60 k microarray and identified the related signal pathways using the Ingenuity IPA program. The gene discussed is TNFSF10; the disease is infectious otitis media.