VIM and esophageal squamous cell carcinoma: To determine whether EMT process is involved in the si-SNHG16-mediated weaken migration capacity of ESCC cells, we performed western blot and immunofluorescence assay to measure the expression levels of epithelial protein markers (E-cadherin and β-catenin) and the expression levels of mesenchymal markers (N-cadherin and vimentin) in cells transfected with si-SNHG16.