If patients show symptom of relapse or disease progression after infusion of cryopreserved donor peripheral blood stem cells, and the class II GVHD complications did not appear, according to the patients’ will, we can again collect the peripheral blood stem cell donors with granulocyte colony sampling factor mobilization of 3∼4 days, the blood cell separator was used to collect a peripheral blood stem cells, and the mononuclear cells and CD34+ cells was counted. The gene discussed is CD34; the disease is graft versus host disease.