To determine the biological function of SOX9 in glioma stemness, we applied shRNAs against SOX9 to U251 and U87MG cells, and found that SOX9-shRNAs significantly decreased SOX9 protein expression in U251 (Figure 3A and 3B) and U87MG cells (Figure 3C and 3D). This evidence concerns the gene SOX9 and glioma.