To determine if hepatic expression of PEMT and the PC/PE ratio are also aberrantly elevated in NAFLD steatosis patients, we measured mRNA levels of PEMT, AHR, and SHP in liver samples from 15 normal individuals, 15 simple steatosis patients, and 15 severe NASH-fibrosis patients. This evidence concerns the gene PEMT and metabolic dysfunction-associated steatotic liver disease.