Univariate analysis identified the following factors significantly associated with poor OS: LMR < 4.01, AFP ≥ 400 ng/mL, BCLC stage B or C, multiple tumors, tumor size ≥ 5 cm, incomplete tumor capsule, AST ≥ 80 U/L, NLR ≥ 2.78 and PLR ≥ 99.5. The gene discussed is AFP; the disease is neoplasm.