SIRT5 and colorectal carcinoma: Next, the addition of the outputs of glutaminolysis, including dimethyl-glutamate (DM glutamate; a cell permeable form of glutamate), dimethyl-α-KG (DM α-KG, a cell permeable form of α-KG), or NEAA mixture (aspartate and asparagine, which were upregulated robustly upon SIRT5 overexpressed) increased the survival of CRC cells, confirming that glutaminolysis plays a critical role in CRC cells’ proliferation (Fig. 5g).