Furthermore, we analyzed the expression of NCK1-AS1 and NCK1 in five cancers (Thyroid carcinoma, Kidney Chromophobe, Adrenocortical carcinoma, Breast invasive carcinoma, Cervical squamous cell carcinoma, and endocervical adenocarcinoma) using TCGA sequencing data sets, showing that NCK1-AS1 is up-regulated in CC tissues (Fig. 3a). Here, NCK1 is linked to thyroid gland carcinoma.