The correlation between the NCK1-AS1 expression and these transcription factors E2F1, XBP1 and SP1 were performed and correlation analysis revealed that NCK1-AS1 has a significantly positive correlation with SP1 in TCGA CESC Tumor data set (Fig. 4d and Supplementary Fig.1C and 1D). The gene discussed is E2F1; the disease is cervical squamous cell carcinoma.