EZH2 and lentivirus infection: The EZH2 expression level and methylation of H3K27 were verified by western blots, and the results showed that EZH2 protein levels and H3K27me3 were significantly reduced after shEZH2-1 lentivirus infection, while the knockdown efficiency of shEZH2-2 was less than satisfactory (Fig. 3a).