Our data showed that there was a higher proportion of CD3+CD8+ EGFR-CAR T cells (54.91%) compared with UTD cells (34.88%), indicating that the cytotoxic T lymphocyte (CTL) population increased significantly after co-culture of these EGFR-CAR T cells with tumor cells (Fig. 2a and Figure S1). This evidence concerns the gene CD8A and neoplasm.