Our data showed: (i) the piggyBac transposon-based gene transduction was effective; (ii) these EGFR-CAR T cells possessed a higher proportion of CD3+CD8+ CTL population with the proliferative ability; and (iii) in vitro- and in vivo-specific and potent cytotoxicity against NSCLC cells. The gene discussed is CD8A; the disease is non-small cell lung carcinoma.