Up-regulation of β-Catenin could rescue the decreased expression of the downstream genes of Wnt signaling pathway in ICC cells suppressed by knockdown of OPN (Fig. 5a), and enhanced in vitro cell proliferation (Fig. 5b), colony formation (Supplementary Figure 5A), migration (Supplementary Figure 5B), and invasion (Fig. 5c) of ICC cells inhibited by OPN knockdown. The gene discussed is SPP1; the disease is intrahepatic cholangiocarcinoma.