GAPDH and hepatocellular carcinoma: A total of 28 HCC tissues were subjected to immunohistochemistry (IHC) of 14-3-3ζ and were divided into 14-3-3ζhigh group (n = 14) and 14-3-3ζlow group (n = 14) (cut-off by median 14-3-3ζ expression level—relative integrated optical density (IOD) value of 14-3-3ζ assayed by western blot compared to that of GAPDH) (Fig. 2a).