MUC1 and neoplasm: We further tested the cytolytic activity of the primed T cells against multiple HLA-A2+ tumor cells lines (breast MDA-MD-231 and MCF-7 and renal A-498) and an HLA-A2- cell line (SK-OV-3), all of which are MUC1+ (Supplemental Figure 3), using a standard chromium release assay.