Nine stem cell marker genes were expressed in the 3D stem-cell condition (SOX2, MYC, MYCN, GATA3, LIN28A, LIN28B, JAK2, CD44, PROM1/CD133), 11 stem cell marker genes were expressed in the 3D serum-containing condition (OCT4/POU5F1, NANOG, KLF4, ALDH1A1, WNT1, STAT3, JAK2, CD44, PROM1/CD133, BMP7, SCA1/ATXN1), whereas only one stem cell marker gene was expressed in the 2D conditions (FZD7, BMI1) (purple blocks), indicating that stemness-related genes were upregulated in the 3D NCP condition and could be involved in the formation of tumor cell aggregations. The gene discussed is POU5F1; the disease is neoplasm.