While CD24 and CD44 were expressed at relatively similar proportions by CRC liver metastases organoids, CD133 and CD166 were only abundantly expressed by LM7727, LM8794 and LM8878, cultures which showed poor expression of the differentiation marker, CEA CAM5 by immunofluorescence, and had decreased mRNA expression of the differentiation marker, CDX1 when compared to patient samples. The gene discussed is PROM1; the disease is colorectal carcinoma.