To evaluate the relationship between the expression patterns of the PD-1 axis and survival in osteosarcoma, we first analyzed OS in the GSE21257 dataset (53 cases) and found that PD-L1 expression had a negative correlation tendency with OS (P = 0.077), while PD-1 expression had no correlation with OS (P = 0.749). Here, CD274 is linked to osteosarcoma.