Positive controls were human seminoma for OCT4 (Figure S5A in Supplementary Material, pink, arrows), normal skin for SOX2 (Figure S5B in Supplementary Material, pink, arrows), seminoma for NANOG (Figure S5C in Supplementary Material, pink, arrows), breast cancer for KLF4 (Figure S5D in Supplementary Material, pink, arrows), and normal prostate for c-Myc (Figure S5E in Supplementary Material, pink, arrows). The gene discussed is KLF4; the disease is seminoma.