KL and ischemia: As shown in Figure 1, transient forebrain ischemia and reperfusion did not markedly affect Klotho protein or mRNA levels in the LV-GFP-treated 2VO group compared with sham-operated controls (p > 0.05), whereas LV-KL strongly upregulated Klotho expression in both the choroid plexus and ischemic brain (p < 0.01, vs. LV-GFP group).