HSP27 was significantly overexpressed in HT-29 colon cancer cells early after exposure to hyperthermic chemotherapy with 5-FU compared with normothermic controls and tumor cells without chemotherapy (0.5 hours after 41°C: FD 3.8 and 43°C: FD 11.7 versus controls without cytostatic treatment 41°C: FD 1.5 and 43°C: FD 1.5) (Figure 5, top). This evidence concerns the gene HSPB1 and malignant colon neoplasm.