Here, we demonstrated for the first time that colon cancer cells exposed to cytostatic treatment with chemotherapeutic substances for HIPEC (MMC and OXA) and for systemic chemotherapy (5-FU) are highly sensitive for upregulated expression of all 3 HSPs, HSP27, HSP70 and HSP90, when imitating clinically HIPEC conditions. This evidence concerns the gene HSPB1 and colonic neoplasm.