MKI67 and neoplasm: After 24 hours and more vigorously after 48 hours of initial normothermic and hyperthermic incubation with all 3 cytotoxic substances, HT-29 tumor cells demonstrated significantly upregulated Ki-67 expression (5-FU 37°C: FD 4.2 and 8.0, 41°C: FD 4.5 and 11.8, and 43°C: FD 3.6 and 11.1, respectively; MMC 37°C: FD 3.4 and 7.2, 41°C: FD 8.6 and 7.5, and 43°C: FD 5.4 and 6.9, respectively; OXA 37°C: FD 3.3 and 5.1, 41°C: FD 2.2 and FD 4.1, and 43°C: FD 2.1 and 4.2, respectively (Figure 7B)).