HSP27, HSP70, and HSP90 Western blot analysis was performed in 3 human colon cancer cell lines (HT-29, SW480, and SW620) 24, 48, and 72 hours after exposure to hyperthermia with or without additional chemotherapy using 5-FU, MMC, and OXA. This evidence concerns the gene HSPA1A and malignant colon neoplasm.