HSPB1 and cancer: No changes in HSP27 gene expression were detected 24 hours after hyperthermic chemotherapy, whereas 48 and 72 hours after incubation, significantly upregulated expression was again observed in HT-29 cancer cells treated with 5-FU at normothermic and hyperthermic conditions (37°C: FD 5.3 and FD 2.6, 41°C: FD 6.6 and FD 4.4, and 43°C: FD 5.3 and FD 5.7, respectively) (Figure 5, top).