We histologically assessed the severity of liver injury with the NAFLD Activity Score (NAS); whereas 20 weeks of CANA treatment did not affect the steatosis score in WD-fed MC4R-KO mice (Supplementary Figure 3), the scores for lobular inflammation and hepatocyte ballooning were reduced by CANA treatment. This evidence concerns the gene MC4R and metabolic dysfunction-associated steatotic liver disease.