In addition, single-cell-derived clones from the original resistant cell pool revealed that this RPS3a crRNA always co-appeared with the XPO1C528 crRNA (Supplementary Fig. 14a, b), suggesting that these cells were transduced with two lentiviral particles and that decreasing the multiplicity of infection (MOI) will reduce the false-positive rate. The gene discussed is RPS3A; the disease is infection.