As with the H&E and Masson’s staining, Western blot also showed that BDL induced cholestatic hepatic fibrosis by increasing FN (0.16 ± 0.05 vs 0.28 ± 0.14, P < 0.05) and collagen III (0.17 ± 0.02 vs 0.38 ± 0.06, P < 0.05) expression (Fig. 2a-c). The gene discussed is FN1; the disease is Hepatic fibrosis.