To further investigate the effects of miR-181b on taurocholate induced-AP, we injected AdCMV-miR-181b into rats by tail intravenous administration, followed by intraperitoneal taurocholate challenge in 10 days, and found that the overexpression of miR-181b inhibited Beclin1 and LC3-II expression, reduced autophagy, and inhibited pancreatic damage in rats with taurocholate-induced AP. This evidence concerns the gene BECN1 and alkaline phosphatase measurement.