Data for NURR1 mRNA levels compared with vehicle controls, as displayed in Figure 7B, exhibit clear 1,25D dose-dependency for a 3- to 4-fold induction of NURR1 by the hormonal vitamin D. We followed up these mRNA studies by investigating the effect of a single dose of DHA or curcumin treatment using both immunofluorescence as well as Western blotting on the protein expression of NURR1 as well as filaggrin, another biomarker of skin inflammatory disease. The gene discussed is FLG; the disease is inflammatory skin disease.