By D6 PI, concomitant with early glomerulosclerosis and albuminuria, we observed increases in canonical Notch pathway transcripts, Notch1, Nrarp, and Hey2. Increased levels of other Notch basic helix loop helix (bHLH) transcription factors Hes1, Hes3, Hes5, and HeyL were also observed in primary mutant podocytes but not in controls (Figure 3a), although these changes did not reach statistical significance. Here, HES5 is linked to glomerulosclerosis.