MYC and hepatocellular carcinoma: Additionally, we quantified the c-MYC mRNA levels in those HCC cell lines using a quantitative real-time PCR assay, and the results showed that the cell lines with the TT genotype (Hep3B and SMMC-7721) had higher c-MYC mRNA levels compared with the cell line with the GT genotype (HepG2); moreover, the cell lines with the GG genotype (MHCC97H and HCCLM3) had the lowest c-MYC mRNA level (Supplementary Fig. 1b) of the five cell lines.