Multivariate Cox regression analysis showed that KIF4A expression (HR = 1.147, P = 0.001), age (HR = 2.265, P = 0.0336), AFP (HR = 1, P = 0.004), AST (HR = 1.025, P < 0.001), bilirubin (HR = 1.069, P = 0.006), HCC differentiation (HR = 0.321, P = 0.009) and TNM stage (HR = 2.043, P < 0.001) were independent predictors of survival in HCC patients (Table 2). The gene discussed is AFP; the disease is hepatocellular carcinoma.