To confirm regulation of CrebA by the Toll and Imd pathways, we quantified CrebA expression by RT-qPCR 12 h after infection with P. rettgeri and E. faecalis in wildtype (WT) flies, flies deficient for the Imd pathway (RelE20), and flies deficient for the Toll pathway (spzrm7) (Fig 5C). This evidence concerns the gene TLR4 and infection.