We then performed bisulfite sequencing PCR combined with HiSeq sequencing (HiSeq-BSP) (Gao et al. 2014; Mensaert et al. 2014) and nine genes (MTMR1, HAUS7, FAM127C, AIFM1, PIM2, TWIST2, IRAK1, BCAP31, and SOX3) were successfully cloned to validate these DMRs in another six pairs of piglets (IUGR/NBW pair 5–10). The gene discussed is AIFM1; the disease is fetal growth restriction.