Temporal analysis of grooming behavior over the 20 min trail demonstrated that Ulk4+/tm1a female failed to increase grooming activity in the last 10 min of the trial when compared to other groups (WT female: 31.63 ± 4.10 s vs. Ulk4+/tm1a female: 19.86 ± 3.18 s; p < 0.05), and also showed lower rearing activity than WT female (Fig. 2c), suggesting that Ulk4+/tm1a females fail to acclimatize the novel arena possibly due to enhanced anxiety-related behavior. This evidence concerns the gene ULK4 and Anxiety.