There were no significant differences in the levels (%) of T effector (CD4+, CD8+), T regulatory (FOXP3+, CTLA-4+, PD1+) and NK (CD56+) cells in the tumour-free para-cortical compartments of metastatic ALNs and non-metastatic ALNs (Table 4). This evidence concerns the gene NCAM1 and neoplasm.