In the present study, we evaluated the stability of six candidate reference genes (HPRT, β-actin, Sdha, GAPDH, 18S and cyclophilin) in two ischemia models: N2a cells at several time points after OGD/R injury and the intraluminal filament model of MCAO in C57 mice. This evidence concerns the gene HPRT1 and ischemia.