To determine whether constitutive activation of PI3K/AKT and MAPK pathway prevents the inhibitory effect of DHA in PDGFRα-positive ovarian cancer, A2780 cells were transiently transfected with constitutively active AKT (Myc-tagged CA-AKT) expressing vector or/and constitutively active K-RAS (GFP-tagged K-RAS G12V) vector (Supplementary Figure S4A). Here, KRAS is linked to ovarian carcinoma.