In this assay, we cultured E0771-LG cancer cells expressing red fluorescent protein (target) with splenic CD8+ T cells that were preincubated with anti-CD3/CD28 antibodies (effector), and detected tumor cell apoptosis indicated by green fluorescence from a fluorogenic caspase-3 substrate under the microscopy (Figure 5A). Here, CASP3 is linked to neoplasm.