MAPT and Alzheimer disease: To determine the potency of the AD p-Tau to template the host Tau into NFTs in 3 × Tg-AD mice, we unilaterally injected 0.35 μg of the oligomeric Tau protein into the right hippocampus of 11- to 12-month-old female 3 × Tg-AD mice and evaluated Tau pathology at 3 and 6 weeks after the injection (Fig. 1a).