Intriguingly, neurons with templated Tau pathology in 13.5-month-old 3 × Tg-AD mice showed even much higher, approximately twofold, load of tau inclusions compared with the spontaneously developed Tau pathology in 18-month-old naive female 3 × Tg-AD mice (Fig. 2e and f). This evidence concerns the gene MAPT and Alzheimer disease.