TYR and melanoma: As shown in Figure 6, after treatment with PN3 at concentrations of 25, 50, and 100 μg/mL, TYR expression in B16 melanoma cells was significantly reduced by 46.64%, 57.25%, and 78.85%, respectively, in a dose-dependent manner, and the expression levels of TRP-1 were decreased by 56.90%, 59.35%, and 86.99%, respectively.