In agreement with these in vitro growth assays, RKO cells with stable expression of RIOK1-K411R and RIOK1-K411R/T410E showed greater tumor growth than cells with RIOK1-WT in a xenograft tumor model, while the stable expression of RIOK1-T410A stable obviously retarded CRC growth (Figure 9C and D). Here, RIOK1 is linked to colorectal carcinoma.