To explore the functional significance of both methylation and phosphorylation of RIOK1, we evaluated the impact of an unmethylatable mutation K411R, a non-phosphorylatable mutation T410A, and an unmethylatable plus phosphomimetic mutation (K411R/T410E) of RIOK1 on CRC cell proliferation, migration, invasion, tumor growth and metastasis. This evidence concerns the gene RIOK1 and colorectal carcinoma.