IHC demonstrated that CRC tissues showed higher expression of RIOK1 compared to matched normal tissues (Figure 1C1), and that the percentage of cells expressing RIOK1 were 25%, 52.2%, 67.7%, and 87.8% in cancer stage I, II, III, and IV of CRC, respectively (Figure 1C2), revealing that RIOK1 expression correlates with CRC malignancy. The gene discussed is RIOK1; the disease is cancer.