In parallel, E-cadherin and vimentin, which may account for reversal of epithelial-mesenchymal transition (EMT), were down-regulated and up-regulated respectively in inhibitors-treated cells (Figure 2—figure supplement 2B), further demonstrating the involvement of PI3K/AKT cascade in the regulation of CRC and GC cells by RIOK1. This evidence concerns the gene AKT1 and gastric cancer.