Dahlmann M et al. have reported that stable knockdown of S100A4 in CRC HCT116 cells by transfection with S100A4‐shRNA expression plasmids resulted in reduced the liver metastatic potential of CRC cells when intrasplenically transplanted in mice.61 Moreover, hydrodynamics‐based systemic treatment with plasmids DNA for S100A4‐specific shRNA, via repeated tail vein injection, inhibited the formation of liver metastases.61 These results suggest that S100A4 may be a critical downstream target of S100P and Trx‐1 and is responsible for the S100P‐ or Trx‐1‐induced EMT and invasiveness of CRC cells. This evidence concerns the gene S100P and colorectal carcinoma.