APC and colorectal carcinoma: However, the functional destruction complex has been usually disrupted in APC-mutant CRC cells including HCT-8 cells [47–50], and our present data showed that although DVLs suppressed GSK3β activity in HCT-8 cells, the inactivation of GSK3β did not change the protein level of β-catenin in cytoplasm and nucleus (Supplementary Figure 1).