To assess the effect of GPR48/LGR4 in tumorigenesis of differentiated thyroid carcinoma, we transfected a GPR48/LGR4 expression plasmid into TPC-1 and BCPAP, and investigated growth by CCK-8 viability assay and migration by Transwell assay (Figure 2D-2K). This evidence concerns the gene LGR4 and thyroid gland carcinoma.