To investigate whether miR-33b was involved in the course of CAFs activating lung cancer cell EMT programming, we examined the expression of miR-33b and its post-transcription factors (EMT-TFs), snail homolog 1 (SNAI1), twist basic helix–loop–helix transcription factor 1 (TWIST1) and zinc-finger E-boxbinding homeobox 1 (ZEB1) in lung cancer cell lines co-cultured with CAFs. The gene discussed is TWIST1; the disease is lung cancer.