As a limitation of our study, we have to take into account that ChIP-seq showed 500 bp long DNA binding region for SIRT1 at Mxd1 gene, whereas the ChIP assay verified only a smaller 100 bp DNA sequence in this region and we cannot exclude sub-sequential stronger or transient binding of SIRT1 along the different stages of tumor progression. This evidence concerns the gene SIRT1 and neoplasm.