By ChIP assay, using primers targeting the same Mxd1 sequence found to be enriched in ChIP-seq (Figure 3B, primer sequences highlighted in orange), we showed that SIRT1 was more associated with Mxd1 in melanoma cell lines (non-metastatic 4C11- and metastatic 4C11+ cells) compared to non-tumorigenic ones (melan-a melanocytes and pre-malignant 4C melanocytes) (Figure 4B). The gene discussed is SIRT1; the disease is melanoma.