In the present study, we examined the effects of NaHS for 8 weeks on 5/6 Nx rats and found that H2S treatment attenuated MDA levels and NF-κB activity, and increased SOD activity at week 12 after induction of 5/6 nephrectomy, suggesting its long-term renoprotective effects against CKD. This evidence concerns the gene NFKB1 and chronic kidney disease.