Within avulsed MNs, infection with AAVrh10-GFP did not change the nuclear ring-like pattern of SIRT1 expression induced by RA, but infection with AAVrh10-SIRT1 led to accumulation of SIRT1 predominantly in the cytoplasm of MNs, similar to NeuroHeal’s effect (Fig. 7A). Here, SIRT1 is linked to infection.