SMOX and infection: The strain mimicking a constitutively unphosphorylated state of AlgR, PAO1 algR D54N, was defective for virulence relative to the parental PAO1 strain, with 57% survival (P = 0.0002, log rank test), indicating that AlgR phosphorylation is critical for the full virulence of P. aeruginosa in a Drosophila feeding infection model (Fig. S6A).