In these experiments, we modulated the balance of arachidonic acid metabolism by incubating the cells with indomethacin (a nonselective cyclooxygenase 1/2 inhibitor), and we investigated the effects of this inhibitor on PI3K-Akt-mTOR signaling in primary AML cells derived from five patients showing constitutive signaling throughout this pathway. This evidence concerns the gene AKT1 and acute myeloid leukemia.